Aufsatz
Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils
Zusammenfassung
The increased use of cereal/legume crop rotation has been advocated as a strategy to increase cereal yields of subsistence farmers in West Africa, and is believed to promote changes in the rhizosphere that enhance early plant growth. In this study we investigated the microbial diversity of the rhizoplane from seedlings grown in two soils previously planted to cereal or legume from experimental plots in Gaya, Niger, and Kaboli, Togo. Soils from these legume rotation and continuous cereal plots were placed into containers and sown in a growth chamber with maize (Zea mays L.), millet (Pennisetum glaucum L.), sorghum (Sorghum bicolor L. Moench.), cowpea (Vigna unguiculata L.) or groundnut (Arachis hypogaea L.). At 7 and 14 days after sowing, 16S rDNA profiles of the eubacterial and ammoniaoxidizing communities from the rhizoplane and bulk soil were generated using denaturing gradient gel electrophoresis (DGGE). Community profiles were subjected to peak fitting analyses to quantify the DNA band position and intensities, after which these data were compared using correspondence and principal components analysis. The data showed that cropping system had a highly significant effect on community structure (p <0.005), irrespective of plant species or sampling time. Continuous cereal-soil grown plants had highly similar rhizoplane communities across crop species and sites, whereas communities from the rotation soil showed greater variability and clustered with respect to plant species. Analyses of the ammonia-oxidizing communities provided no evidence of any effects of plant species or management history on ammonia oxidizers in soil from Kaboli, but there were large shifts with respect to this group of bacteria in soils from Gaya. The results of these analyses show that crop rotation can cause significant shifts in rhizosphere bacterial communities.
Zitierform
In: Biology and Fertility of Soils. Berlin : Springer. 37.2003, H. 2, S. 73-82Sammlung(en)
Publikationen (Fachgebiet Ökologischer Pflanzenbau und Agrarökosystemforschung in den Tropen und Subtropen)Zitieren
@article{urn:nbn:de:hebis:34-2007050918098,
author={Alvey, S. and Yang, C.-H. and Bürkert, Andreas and Crowley, D. E.},
title={Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils},
year={2003}
}
0500 Oax 0501 Text $btxt$2rdacontent 0502 Computermedien $bc$2rdacarrier 1100 2003$n2003 1500 1/eng 2050 ##0##urn:nbn:de:hebis:34-2007050918098 3000 Alvey, S. 3010 Yang, C.-H. 3010 Bürkert, Andreas 3010 Crowley, D. E. 4000 Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils / Alvey, S. 4030 4060 Online-Ressource 4085 ##0##=u http://nbn-resolving.de/urn:nbn:de:hebis:34-2007050918098=x R 4204 \$dAufsatz 4170 7136 ##0##urn:nbn:de:hebis:34-2007050918098
2007-05-09T09:24:06Z 2007-05-09T09:24:06Z 2003 1432-0789 0178-2762 urn:nbn:de:hebis:34-2007050918098 http://hdl.handle.net/123456789/2007050918098 291294 bytes application/pdf eng Urheberrechtlich geschützt https://rightsstatements.org/page/InC/1.0/ Crop rotation DGGE Plant nutrition Rhizosphere Soil microbiology 630 Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils Aufsatz The increased use of cereal/legume crop rotation has been advocated as a strategy to increase cereal yields of subsistence farmers in West Africa, and is believed to promote changes in the rhizosphere that enhance early plant growth. In this study we investigated the microbial diversity of the rhizoplane from seedlings grown in two soils previously planted to cereal or legume from experimental plots in Gaya, Niger, and Kaboli, Togo. Soils from these legume rotation and continuous cereal plots were placed into containers and sown in a growth chamber with maize (Zea mays L.), millet (Pennisetum glaucum L.), sorghum (Sorghum bicolor L. Moench.), cowpea (Vigna unguiculata L.) or groundnut (Arachis hypogaea L.). At 7 and 14 days after sowing, 16S rDNA profiles of the eubacterial and ammoniaoxidizing communities from the rhizoplane and bulk soil were generated using denaturing gradient gel electrophoresis (DGGE). Community profiles were subjected to peak fitting analyses to quantify the DNA band position and intensities, after which these data were compared using correspondence and principal components analysis. The data showed that cropping system had a highly significant effect on community structure (p <0.005), irrespective of plant species or sampling time. Continuous cereal-soil grown plants had highly similar rhizoplane communities across crop species and sites, whereas communities from the rotation soil showed greater variability and clustered with respect to plant species. Analyses of the ammonia-oxidizing communities provided no evidence of any effects of plant species or management history on ammonia oxidizers in soil from Kaboli, but there were large shifts with respect to this group of bacteria in soils from Gaya. The results of these analyses show that crop rotation can cause significant shifts in rhizosphere bacterial communities. open access In: Biology and Fertility of Soils. Berlin : Springer. 37.2003, H. 2, S. 73-82 Alvey, S. Yang, C.-H. Bürkert, Andreas Crowley, D. E. The original publication is available at www.springerlink.com
Die folgenden Lizenzbestimmungen sind mit dieser Ressource verbunden: